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Lynch Syndrome and BRCA 1 2 PowerPoint Presentation

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Description : Check out this medical presentation on Familial Pancreatic Cancer, which is titled "Lynch Syndrome and BRCA 1/2", to know about Lynch Syndrome and BRCA 1/2 with the help of case presentations.

Tags : Familial Pancreatic Cancer, Lynch Syndrome, BRCA 1/2

Published on : May 18, 2021 | Views : 2411 | Downloads : 4

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Slide 1-

Lynch Syndrome and BRCA 1/2
Slide 2-

21 yo female px to GP with 3/52 of severe abdominal pain colicky in nature with no relieving sx Associated with nausea and reduced appetite 2 presentation to ED with nil Ix PMHX: Asthma Social HX: Smoker Works as an retail assistant Family Hx: Nil known Case 1
Slide 3-

CT Abdo: Suspicious mass colon with lymphadenopathy Colonoscopy: hepatic flexure lesion with biopsy proven malignancy Laparotomy: T4aN0 Adeno CA Bowel penetrates to the surface of the visceral peritoneum with micro- perforation Stage IIB MSI H Mx?
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35 year old female Px for review following WLE and SNB after px with a self detected breast mass PMHX: G2P1 Social Hx: Teacher Ex-smoker Family Hx: Mother Breast CA 50 Aunty ovarian CA 60s Case 2
Slide 5-

Histology Grade 3 15 mm Triple negative IDC Margins clear ½ SN Ki 67 50% Axillary clearance 2/12 LN Commenced on adjuvant chemo therapy Referred to Familial Cancer clinic Found to have a BRCA 1 mutation MX? Case 2
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Approximately 5% of CRC cancers are hereditary LS most common of the inherited colon cancer susceptibility syndromes Increase risk malignancy CRC, Endometrial CA gastric ovarian pancreases urinary tract biliary tract brain small intestine skin Lynch Syndrome (LS)
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Early age of onset CA Multiplicity of cancers Synchronous colorectal cancers Metachronous colorectal cancer Proximal location in the right colon Improved stage-independent survival relative to sporadic CRC Characteristics
Slide 8-

Mucinous histology Poorer differentiation Medullary growth pattern Presence of tumor infiltrating lymphocytes Classic Histology CRC
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2-5% endometrial carcinomas Classically Endometrioid histology similar to sporadic endometrial cancer. serous carcinoma, clear cell carcinoma, uterine malignant mixed mullerian tumors Early stage Favorable prognosis Endometrial cancer
Slide 10-

Autosomal dominant Inherit a germ line mutation in one of several DNA mismatch repair (MMR) genes. Lynch syndrome Ahnen -2013
Slide 11-

Proofreads DNA for mismatches generated during DNA replication MMR inactivation → ↑ mutation rate in dividing cells → ↑tumorigenesis ? Role of Mismatch repair (MMR) genes
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Microsatellites short repetitive DNA sequences Defective MMR G→ abnormalities in the length of microsatellites = microsatellite instability (MSI) Cancers > 40% microsatellite variations = high frequency MSI (MSI High) MSI High molecular signature of Lynch-associated cancers MMR Genes and MSI
Slide 13-

Inherit one abnormal allele MLH1 MSH2 MSH6 PMS2 70% MLH1 MSH2 EPCAM Epigenetic silencing of MSH2 Defective MMR Inactivation of both alleles MMR gene Second allele though mutation, loss of heterozygosity, epigenetic silencing by promoter hypermethylation. Lynch syndrome
Slide 14-

Germ- line Vs Sporadic 7.2-15% Lynch syndrome Reminder Sporadic epigenetic silencing of the promoter region of MMR genes predominantly MLH1 dMMR
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1)Test the tumour Microsatellite instability or immunohistochemistry of MMR genes IHC Sensitivity 83%, Specificity 89% MSI Sensitivity 77-89%, Specificity 90% 2)? Sporadic BRAF V600E mutation , Germline wildtype 3) Genetic Testing Testing for Lynch Syndrome
Slide 16-

Lynch Syndrome ? Who to screen
Slide 17-

Up to 50% of patient with LS fail to meet the revised Bethesda(NCCN guidelines) Who to test?
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Revised Bethesda Criteria CRC diagnosed in patients younger than 50 years of age Presence of synchronous or metachronous colorectal or other LS related tumors regardless of age CRC with histology diagnosed in a patient <60 years old CRC in a patient with one or more first degree relatives with a less related cancer, with one of the cancers occurring under the age of 50 CRC diagnosed in a patient with two or more first or second degree relatives with LS related cancers regardless of age Who to test?
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Endometrial ca Who to Test?
Slide 20-

Patient: Cancer Risk Prognosis Adjuvant treatment Risk to family members Insurance Implications for a diagnosis of Lynch Syndrome
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Cancer Risk in Lynch Syndrome
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NCCN Guidelines Cancer risk up to 70
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MMR defects and Adjuvant Chemotherapy
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Fluoropyrimidine-based adjuvant systemic therapy Clearly shown to have benefit in patients with stage III CRC Stage II colon cancer controversial dMMR predictive lack of benefit to single agent fluoropyrimidine-based chemotherapy ? Difference dMMR germ-line mutations vs sporadic .(Sinicrope FADNA mismatch repair status and colon cancer recurrence and survival in clinical trials of 5-fluorouracil-based adjuvant therapy. J Natl Cancer Inst 2011 Jun 8;103(11):863-75. + MMR defects and Adjuvant Chemotherapy CRC
Slide 25-

Rx similar MMR defects and Adjuvant Chemotherapy Endometrial cancers
Slide 26-

Surveillance for patients with Lynch Syndrome
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CRC Colonoscopy to begin 20-25y or 2-5 years younger than the youngest family diagnosis If MSH6 can start later 30y Endometrial /Ovarian Education to enhance recognition of relevant symptoms No evidence for routine screening Annual endometrial sampling is an option TVU + Ca 125 not recommend, insensitive and not specific Consider TAH/BSO Urotherial CA in MLH1 Annual urinalysis from 25y No clear endience for screening for gastric, duodenal small bowel Surveillance (NCCN)
Slide 28-

CAPP2 861 with LS 600mg Aspirin vs placebo for up to 4 years Median FU of >4 years People taking Aspirin for >2 years 59% reduction in the incidence of CRC HR.49 95%CI .19- 1.86 p=.02 Problems with the study More studies required Aspirin however generally recommended Prevention
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Family history breast and/or ovarian cancer is common BUT <10 % breast cancers < less than 15 % of ovarian cancers associated with germline (inherited) genetic mutations Hereditary Breast and Ovarian CA
Slide 30-

BRCA 1/2
Slide 31-

Characteristics of BRCA 1/2
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Multiple cases w/i same family Early age of onset Bilateral breast CA Synchronous cancers Associated malignancies in patient/family members Male Breast CA Characteristics of BRCA 1/2
Slide 33-

Breast CA BRCA 1 Grade 3 Triple negative (80%) Little DCIS, ↑incidence medullary cancer BRCA 2 no defining histological features Ovarian Most serous papillary ovarian ca 85% Vs in sporadic 40% Characteristics of BRCA 1/2 Classic Histological features
Slide 34-

Tumor suppressor genes Play a number of roles in the maintenance of genome integrity DNA repair Regulation of cell cycle check points Homologous recombination BRCA 1 located on chromosome 17q21. BRCA2 gene located on chromosome 13q What are BRCA 1 and 2?
Slide 35-

Mutation inherited in Autosomal Dominant Incomplete penetrance >1000 different mutations reported lead to a shortened abnormal protein when translated. ↑genomic instability and tumorigenesis Prevalence .25 % general population European ancestry 2.5 % Ashkenazi Jewish ancestry BRCA 1 and 2
Slide 36-

Cancer Risk with BRCA 1/2
Slide 37-

NCCN Common CA: Breast, ovarian, prostate ad pancreatic cancer
Slide 38-

BRCA 1 cervix, uterus, fallopian tube, primary peritoneum, pancreas, esophageal, stomach, and prostate cancers BRCA2 stomach, gallbladder, bile duct, esophagus, stomach, fallopian tube, primary peritoneum, and skin Other CA
Slide 39-

Key differences Difference: Male ca and pancreatic >BRCA 2 Ovarian < BRCA 2 NB Changes in BRCA not seen in sporadic breast CA Sporadic BRCA mutations seen in ovarian CA BRCA 1 vs 2
Slide 40-

Testing
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1)Computer scoring system >10% BOADICEA ,BRCAPRO Manchester score >16 2)Who are obligate carriers 3)Triple negative BC < age 40 yrs 4)Grade 2-3 invasive non-mucinous ovarian, fallopian tube or primary peritoneal cancer < age 70 yrs 5)Invasive non-mucinous ovarian, fallopian tube or primary peritoneal cancer at any age a family history of breast or ovarian cancer Or a personal and/or family history* of breast and/or ovarian cancer, from a population where a common founder mutation exists 6)Where a known pathogenic mutation has been identified (Predictive testing) Genetic testing Who to Test ? (EVIQ)
Slide 42-

Dx Breast CA < 45y Non-mucinous Ovarian, fallopian tube or primary peritoneal cancer Grade 2-3 2 Breast Primaries synchronous or asynchronous 1 dx < 50y Breast CA< 50y with a relative with breast CA Breast CA Dx at any age with a relative with Breast <50 Dx any age with 2 or > relatives with Breast CA or 1 with ovarian Dx at any age with 2 more relatives wit pancreatic CA or prostate CA Triple –ve Breast CA <60 Close male relative with Breast CA If in doubt refer to a FCC ? When to refer to FCC for Screening
Slide 43-

Family Genetic counseling Surveillance Prevention Risk Reduction surgery Consider chemoprevention MX post Dx (NCCN)
Slide 44-

Surveillance
Slide 45-

Breast Awareness stating at 18y Clinical breast exam every 6-12/12 from 25y Breast screening Annual from 25y Or individualized based on earliest age of onset 25-29y MRI or Mammogram (If MRI unavailable) >30-75y Mammogram + MRI annual >75y in individual basis NCCN Guidelines Hereditary Breast/Ovarian CA 2014 Female Surveillance Guidelines
Slide 46-

Breast self exam and education from 35y Clinical breast exam every 6-12/12 from 35y Baseline mammogram aged 40 + annual mammogram of gyencomastia on baseline study Prostate screening for BRCA2 Other CA Surveillance Men and Woman: Education regarding the signs and sx od CA associated with BRCA Male Surveillance Guidelines
Slide 47-

Bilateral total mastectomy ↓ breast cancer by at least 90% BRCA mutations and high risk Breast CA NCCN: Consider Bilateral mastectomy Prevention Risk reduction surgery
Slide 48-

Reduction salpingo-oophorectomy (RRSO) ↓ risk of ovarian or fallopian cancer by 80% 1-4.3 residual risk of primary peritoneal carcinoma ↓ of breast cancer by approximately 50% NCCN: Recommend salpingo -oophorectomy b/w 35-40y Transvaginal U/S + CA-125 not effective screening Prevention Risk Reduction Surgery
Slide 49-

SERM OCP Chemoprevention
Slide 50-

BRCA 1 and Implications for treatment of Breast and Ovarian CA
Slide 51-

Backbone for systemic therapy in triple-negative breast cancer. Consider platinum Impaired HR→ Impair the cells’ ability to repair DNA cross-links Platinum's alter structure of DNA further by intrastrand adducts and interstrand crosslinks Impede cell division ? Greater efficacy in BRAC mutation carriers Chemotherapy
Slide 52-

PARP1 an important regulator of the DNA base-excision–repair pathway BRCA 1/2 impaired Homologous recombination + PARP inhibitor= synthetic lethality PARP inhibitor
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Mx: Further family hx: Estranged from father Thus unclear family hx BRAF IHC WT Genetic counseling Refused ? Adjuvant Chemo controversial Case 1
Slide 56-

Initial Rx Adjuvant chemo Y AC-T + platinum FEC- D ? RXT Yes Hormone rx No Case 2
Slide 57-

Implication for BRCA 1 Consideration of bilateral mastectomy Recommend salpingo -oophorectomy Genetic counseling for family members Role of PARP inhibitor ?