Download Free Medical Orthopaedic Pathological Specimen and Histology PowerPoint Presentation

Orthopaedic Pathological Specimen and Histology PowerPoint Presentation

DOWNLOAD MEDICAL POWERPOINT PRESENTATION

About This Presentation

Description : Check out this medical presentation on Ewing Sarcoma, which is titled "Orthopaedic Pathological Specimen and Histology", to know about the orthopaedic pathological specimen, related abnormalities, and provisional diagnosis.

Tags : Ewing Sarcoma, Orthopaedic Pathological Specimen, Histology, abnormalities, provisional diagnosis

Published on : May 13, 2021 | Views : 1411 | Downloads : 0

Shareable Url :

Embed Code :

Comment

Transcript

Slide 1-

ORTHOPAEDIC PATHOLOGICAL SPECIMEN AND HISTOLOGY
Slide 2-

DESCRIBING GROSS SPECIMEN A: Identify the part:  Knee / prox. femur/ prox. tibia/ pelvis /scapula.  Epiphyseal / Metaphyseal / Diaphyseal.  Physeal plate visible? – Immature pt.
Slide 3-

B:Describe the abnormality:  Nature: solid / soft & friable /cystic / varigated  Matrix: bony / cartilaginous / fibrous  Secondary changes: central necrosis/ hemorrhage / cystic change  Cortical destruction, periosteal elevation  Intramedullary spread  Tumor margin / capsule: well defined / illdefined  Extension: into soft tissue / joint; invasion / infiltration to surrounding tissue
Slide 4-

C: Give provisional diagnosis.
Slide 5-

Ewing’s sarcoma Myeloma Osteoid osteoma Ewing’s sarcoma Myeloma Fibrous dysplasia Majority Chondroblastoma Giant cell tumor Epiphyseal Chondroblastoma Giant cell tumor Diaphyseal Ewing’s sarcoma Myeloma, Fibro sarcoma, Reticulum cell sarcoma Adamantinoma Fibrous dysplasia Osteoid Osteoma Metaphyseal Most others
Slide 6-

Enchondroma Unicameral bone cyst Round cell tumors Majority Central Enchondroma Unicameral bone cyst Round cell tumors Cortical Osteoid osteoma Eccentric Most other tumors
Slide 7-

ppt slide no 7 content not found
Slide 8-

 Age: 20-40.  Epiphyseal location (After epiphyseal closure)  Common location: around knee (50%), distal radius  Xray: Geographic lytic lesion, thinned & ballooned out cortex. Gross pathology:  Large red - grey - brown tumor  Soft & friable  Areas of cystic degeneration / necrosis & blood filled cavities GIANT CELL TUMOR Incidence : 5% of all bone neoplasm Sex : F:M = 2: 1 Bone Involved : Long tubular bones Distal femur  Prox tibia  Distal radius – UE Humerus  UE fibula  LE Ulna
Slide 9-

Soap bubble appearance
Slide 10-

HISTOPATHOLOGY: •Uniform oval mononuclear stromal cells : mesenchymal origin •Appear to grow in a syncytium •Numerous osteoclast-type giant cells: reactive with centrally placed uniform size nuclei (40-100) •Necrosis, hemorrhage, hemosiderin deposition and reactive bone formation •Relatively few mitotic figures in relation to the dense cellularity of the tumor
Slide 11-

GIANT CELL VARIANTS Includes the tumors which show giant cells in histology-         A. ABC B. Brown tumor C. Chondromyxoid fibroma, chondroblastoma D. Desmoplastic fibroma E. Epulis – Giant cell reparative granuloma F. Fibrous dysplasia, non ossifying fibroma G. Giant cell rich osteosarcoma H. Benign fibrous histiocytoma
Slide 12-

Giant cell…… Physiological: Megakaryocyte Pathological  Langhans : fused epitheliod cells. Peripherally arranged 7-21 nuclei. TB, histoplasmosis, sarcoidosis, other mycobacteria  Foreign body: fused macrophages. Numerous nuclei scattered in cytoplasm      Aschoff : Rheumatic heart disease Reed Sternberg: Hodgkin’s lymphoma Touton : xanthoma, xanthogranuloma, dematofibroma Viral: Warthin Finkeldy (measles), resp syncitial virus, parainfluenza Tumor
Slide 13-

Features of malignant changes : Size and number of giant cells decrease Stroma shows : Heterogenicity as : Crowding, Plumping up, Increased chromatin, Increased mitotic figures. Vascularity increases Grading of GCT (Jaffe): Stromal cells Giant cells Grade-1 or conventional GCT Stroma less Large number Grade-II or Borderline GCT Prominent cellular atypia Decrease in number Grade-III or fully malignant Sarcomatous stroma Sparse
Slide 14-

Enneking’s Staging of Benign GCT Campanacci’s Radiological Grading Grade I – Cystic lesion Grade II – Cortex thin but not perforated Grade III – Tumour extended into surrounding soft tissue
Slide 15-

Treatment (Grade-I and Grade-II) Only curettage. Curettage + bone grafting. Curettage + bone grafting + liquid nitrogen (cryosurgery). Extended aggressive curettage + Phenol / argon beam coagulation / bone cementing. Grade-III – Resection + Reconstructions Resection : En Block (Joints lost) * Role of Radiation in GCT- spine/pelvis (1500-5000 rads for 5-6 wks)
Slide 16-

Giant Cell Tumour
Slide 17-

Giant Cell Tumour
Slide 18-

OSTEOSARCOMA  Bimodal age distribution: Primary 10-20, secondary 50-70.  75% around knee  Bone forming tumor arising from bone  Several subtypes –  By location (Central or Juxtacortical) Degree of differentiation: well to dedifferentiated Histologic variance: osteoblastic/chondroblastic/fibroblastic/telangictatic/small cell  Associated with Retinoblastoma (rb gene), Li Fraumeni syndrome (p53 gene), Rothmund Thomsen syndrome.
Slide 19-

GROSS PATHOLOGY:  Metaphyseal  Tan-white solid tumor fills most of the medullary cavity of the metaphysis and proximal diaphysis  Expanding & infiltrating through the cortex, lifts the periosteum (Codman’s triangle) and forms soft tissue masses on the side of the bone  Areas of hemorrhage and central necrosis.
Slide 20-

HISTOPATHOLOGY:  Pleomorphic and anaplastic cell population- large hyperchromatic nuclei, mitotic figures  Abundant fibrous/ chondroid matrix  Formation of pink homogenous osteoid by neoplastic cell: characteristic.  The neoplastic bone has a coarse, lacelike architecture but is also deposited in broad sheets or as primitive trabeculae.  Osteoblastic, chondroblastic or fibroblastic types  Osteoclast-like giant cells may be present
Slide 21-

Ewing’s Sarcoma
Slide 22-

CHONDROSARCOMA  Age: 30-60  Primary or Secondary ( Enchondromatosis [Ollier 50%, Maffucci 100%], diaphyseal aclasis 20%, osteochondroma 0.25%)  Pelvis (30%), Femur (20%)  Types: Conventional, mesenchymal, clear cell, juxtacotical, dedifferentiated  Most common malignant bone tumor of hand
Slide 23-

GROSS PATHOLOGY:  Large bulky tumor  Made up of lobules of gray-white /somewhat translucent glistening tissue.     Tumor permeating throughout the medullary cavity, growing through the cortex, and forming a relatively well- circumscribed soft tissue mass. At center – necrotic/liquefied/cystic. Gelatinous appearance secondary to myxoid changes in matrix. May show calcification. The adjacent cortex is thickened or eroded, and the tumor grows with broad pushing fronts into the surrounding soft tissue.
Slide 24-

HISTOPATHOLOGY: • Tumor cells produce cartilaginous matrix; well, moderate or poorly differentiated. • May have only minor or focal atypia • Intracytoplasmic hyaline globules common in low grade tumors
Slide 25-

EWING’S SARCOMA James Ewing 1921 GROSS PATHOLOGY:  Diaphyseal in long bones; also pelvis, rib, scapula  White tan grey mass like brain; or red like red currant gelly if hemorrhagic  With necrosis & hemorrhage  Cortical destruction  Invasion to soft tissue, no capsule
Slide 26-

HISTOPATHOLOGY: • Homogenous & densly packed undifferentiated small round blue cells like lymphocytes; regular nuclei, infrequent mitoses, scant clear cytoplasm. • Abundant glycogen: PAS + diastase digestible • Rare Homer- Wright pseudorosettes (7-8 tumor cells arranged in a circle about a central fibrillary space)
Slide 27-

D/D : Small Round Cell Tumor  Ewing’s: mic2 overexpression - CD99/013 . t(11;22)(EWS-FLI-1). NSE +  PNET (Primitive neuroectodermal tumor): CD99/013. S100, chromogranin, synaptophysin; > 20% Homer Wright rosettes  Lymphoma (reticulum cell sarcoma, NHL): CD 45, LCA  Small cell carcinoma: keratin, synaptophysin, chromogranin  Mesenchymal chondrosarcoma  Neuroblastoma: neurofilament. S100, chromogranin, synaptophysin.  Alveolar rhabdomyosarcoma: actin, desmin, vimentin, MyoD1, myogenin  Leukemia
Slide 28-

OSTEOCHONDROMA GROSS PATHOLOGY:  Mushroom shaped  Cartilage-capped bony outgrowth  Cartilage cap usually regular and thin     Sessile / Pedunculated Pedunculated : attached to skeleton by bony stalk; medullary cavity of the osteochondroma and bone are in continuity Diaphyseal aclasis: multiple heriditary exostosis Trevor’s disease: Epiphyseal side osteochondroma Osteochondroma – most common benign bone tumor AGE: during growth period SEX: Male SITE Attachments near metaphysis
Slide 29-

ppt slide no 29 content not found
Slide 30-

ppt slide no 30 content not found
Slide 31-

Painful osteochondroma:  Fracture  Bursitis  Malignant transformation: fluffy calcification in the cartilage cap, thickness of cartilage cap > 1 cm. Radiology Treatment
Slide 32-

CHRONIC OSTEOMYELITIS  Sequestrum is the necrotic bone that is embedded in the pus/infected granulation tissue.  Involucrum is the new bone laid down by the periosteum that surrounds the sequestra.  Cloaca is the opening in the involucrum through which pus & sequestra make their way out.
Slide 33-

Types of sequestrum…..           Tubular: long bone Annular: amputation stump Ring: around pin tracts Flake, coke, rice grain: tubercular Button: histiocytosis Feathery: syphilis Match stick: sickle cell Coloured: fungal Black: gun shot Bombay: exposed bone after open fracture
Slide 34-

TUBERCULAR SPONDYLITIS (POTT’S) SPINE  Paradiscal  Loss of height of vertebra  Caseating necrotic tissue  Bone necrosis; sequestra  Sclerosis
Slide 35-

Acute osteomylitis is most commonly caused by a)staphylococcus aureus b)H. influenzae c)Streptococcus d)Salmonella e) Pseudomonas
Slide 36-

Earliest site of bone involvement in hematogenous osteomyelitis a)Metaphysis b)diaphysis c)Epiphysis d) Physis e)Point of entry of nutrient artery
Slide 37-

Complications of acute osteomyelitis a)malignancy b)fracture of the affected bone c)sepsis d)chronicity
Slide 38-

The most common organism causing osteomyelitis in drug abusers is a)E coli b)pseudomonas c)Klebsiella d)Staph aureus
Slide 39-

Giant cell tumour is located in the? a) Epiphysis b) Metaphysis c) diaphysis d) all of the above
Slide 40-

Soap bubble appearance is seen in which of the following tumours? a) Osteosarcoma b) Chondrosarcoma c) Osteoclastoma d) Ewings sarcoma
Slide 41-

ppt slide no 41 content not found
Slide 42-

A 15 year old boy is injured while playing cricket. X-rays of the leg rule out of a possible fracture. The radiologist reports the boy has an evidence of aggressive bone tumour with both bone destruction and soft tissue mass. The bone biopsy reveals a bone cancer with neural differentiation. Which of the following is the most likely diagnosis? a) Chondroblastoma b) Ewing‘s sarcoma c) Neuroblastoma d) Osteosarcoma
Slide 43-

Both Ewing sarcoma and peripheral neuroepithelioma belong to the Ewing family of tumors and are considered neural tumors . Unlike neuroblastoma, these neural tumors are not derived from the sympathetic system, and catecholamine metabolites are not excreted in the urine . In vitro, these tumors show neural differentiation and have neural features. Results with neuron-specific enolase and S-100 protein testing are positive. electron microscopy reveals neural structures such as neurites and dense-core granules. Glycogen granules are present, and alkaline phosphatase is absent.
Slide 44-

Neuroblastoma (NB) is the most common extracranial solid cancer in childhood and the most common cancer in infancy It is a neuroendocrine tumor, arising from any neural crest element of the sympathetic nervous system (SNS). It most frequently originates in one of the adrenal glands, but can also develop in nerve tissues in the neck, chest, abdomen, or pelvis.
Slide 45-

ppt slide no 45 content not found
Slide 46-

Although it can affect people of all ages, it most often appears in males younger than 25 years old. Biopsy and hematoxylin and eosin (H&E) staining under high light microscopy magnification show numerous chondroblastic cells embedded in a highly mesenchymal cartilaginous matrix. Chicken-wire calcification in a latticelike pattern surrounding the tumor cells is present in 30% of chondroblastomas. CHONDROBLASTOMA
Slide 47-

Malignant tumour of mesenchymal cells characterized by formation of osteoid is description fit for ? a) Osteosarcoma b) Chondrosarcoma c) Osteoclastoma d) Ewings sarcoma
Slide 48-

Secondary osteosarcoma can be caused after? a) Pagets disease b) fibrous dysplasia c) multiple enchondromatosis d) All the above
Slide 49-

Causes of secondary osteosarcoma: 1- Radiation 2- Bone Diseases: a) Pagets disease b)Hereditary multiple osteochondroma c)Enchondromatosis d) Fibrous dysplasia 3- Inherited Cancer Syndromes: a) Li–Fraumeni syndrome b) Rothmund-Thomson syndrome c) Hereditary retinoblastoma d) Bloom syndrome e) Werner syndrome f) Diamond-Blackfan anemia
Slide 50-

Most common site of metastasis in Osteosarcoma is ? a) Lungs b) Heart c) Brain d) Liver
Slide 51-

True statement about involucrum and sequestrum: a) Involucrum is a piece of dead bone b) Sequestrum is a sheath of new bone formation c) Sequestrum is surrounded by involucrum d) Involucrum is surrounded by sequestrum
Slide 52-

Pulsatile tumor is seen in 1. Osteosarcoma 2. Osteoclastoma 3. Ewings 4. Chondrosarcoma
Slide 53-

Pulsating Tumors of the Bone *Primary tumors that may present as pulsating lesions : 1- Telengiectatic Osteogenic sarcoma 2- Angioendothelioma/Angiosarcoma of bone 3- Aneurysmal bone cyst 4- Giant cell tumor (rarely) *Secondaries/Metastases that may present as pulsating lesions : 1- Metastasis from Renal cell carcinoma 2- Metastasis from Thyroid carcinoma
Slide 54-

Ewings sarcoma is believed to arise from : a) Aberrant cartilage rests b) Endothelial cells of bone marrow c) Mesothelial cells d) Periosteocytes
Slide 55-

Which of the following malignant tumours is radio resistant? A. Ewing’s sarcoma B. Retinoblastoma C. Osteosarcoma D. Neuroblastoma
Slide 56-

Most radio resistant tumors are: Malignant melanoma Osteosarcoma Pancreatic carcinoma
Slide 57-

All the statements are true about exostosis, except A. It occurs at the growing end of bone B. Growth continues after skeletal maturity C. It is covered by cartilaginous cap D. Malignant transformation may occur
Slide 58-

Which of the following conditions is least likely to present as an eccentric osteolytic lesion: A. Aneurysmal bone cyst B. Giant cell tumour C. Fibrous cortical defect D. Simple bone cyst
Slide 59-

7 year old child presents with a lesion in upper tibia. X-ray shows radiolucent area with Codman’s triangle and Sunray appearance. Diagnosis is : a) Ewing’s sarcoma b) Osteosarcoma c) Osteoid osteoma d) Chondrosarcoma
Slide 60-

Sunray appearance” on x-rays is suggestive of : 1. an osteogenic sarcoma. 2. a chondrosarcoma. 3. a metastatic tumor in the bone. 4. an Ewing’s sarcoma.
Slide 61-

The Codman triangle may be seen with aggressive lesions: 1- osteosarcoma 2- Ewing sarcoma 3- osteomyelitis 4- active aneurysmal bone cyst 5- giant cell tumour 6- metastasis 7- chondrosarcoma (especially juxtacortical chondrosarcoma) 8- malignant fibrous histiocytoma
Slide 62-

ppt slide no 62 content not found
Slide 63-

Chondroblastomas most commonly occurs in ? a- epiphysis b- diaphysis c- metaphysis d- medullary cavity
Slide 64-

Most common benign tumour of the bone ? a- giant cell tumour b- simple bone cyst c- osteochondroma d- enchondroma
Slide 65-

Onion peel appearance on x ray is seen in? a- osteosarcoma b- ewings sarcoma c- osteoclastoma d- osteochondroma
Slide 66-

Which of the following is not a benign tumour ? a- chondroma b- chordoma c- osteochondroma d- enchondroma
Slide 67-

Most common site of osteogenic sarcoma ? a- femur upper end b- femur lower end c- tibia upper end d- tibia lower end
Slide 68-

Turn - o - plasty is used in the treatment of which of the following tumours ? a- osteosarcoma b- osteoclastoma c- osteochondroma d- osteoblastoma e- chondrosarcoma
Slide 69-

Which of the following is the least commonly affected site for chondrosarcoma? a. Pelvis b. Proximal Femur c. Proximal Humerus d. Hands and Feet
Slide 70-

A 30-year-old man has pain about his left knee that he says has been worsening "for weeks". On physical examination the left knee appears larger than the right, and there is tenderness to palpaption. A radiograph reveals a 7 cm lytic lesion involving the epiphyseal region of the distal femur with a "soap bubble" appearance. The lesion is curretted, and histologically there are numerous multinucleated cells in a stroma with plump to spindle shaped mononuclear cells. Which of the following is the most likely diagnosis? A Osteosarcoma B Chondrosarcoma C Malignant fibrous histiocytoma D Giant cell tumor E Tuberculosis
Slide 71-

THANK YOU