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The Citric Acid Cycle PowerPoint Presentation

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This medical talks about the citric acid cycle, also known as the Krebs cycle or the tricarboxylic acid cycle. The citric acid cycle is a metabolic pathway that occurs in the mitochondria of eukaryotic cells and in the cytoplasm of prokaryotic cells. It is a series of biochemical reactions that oxidize acetyl-CoA, which is derived from the breakdown of carbohydrates, fats, and proteins, to produce energy in the form of ATP.

The citric acid cycle begins with the entry of acetyl-CoA into the cycle, which combines with oxaloacetate to form citrate. Citrate is then metabolized through a series of enzymatic reactions to produce NADH, FADH2, and ATP. The intermediates of the cycle are also used for the synthesis of various biomolecules, including amino acids, nucleotides, and heme.

The citric acid cycle is a central metabolic pathway that links many other metabolic pathways together. It plays a crucial role in the metabolism of carbohydrates, fats, and proteins, and is essential for the production of energy in the form of ATP.

Tags : citric acid cycle, the Krebs cycle, the tricarboxylic acid cycle, metabolic pathway, endocrinology, endocrinology

Published on : Apr 21, 2023 | Views : 1367 | Downloads : 3

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Slide 1-

The Citric acid cycle
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The Citric acid cycle It is called the Krebs cycle or the tricarboxylic and is the “hub” of the metabolic system. It accounts for the majority of carbohydrate, fatty acid and amino acid oxidation. It also accounts for a majority of the generation of these compounds and others as well. Amphibolic - acts both catabolically and anabolically 3NAD+ + FAD + GDP + Pi + acetyl-CoA 3NADH + FADH + GTP + CoA + 2CO2
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History By 1930 it was established that the addition of lactate, acetate succinate, malate, a-ketoglutaric acid (dicarboxylic acids) and citrate and isocitrate (tricarboxylic acids) when added to muscle mince that they stimulated oxygen consumption and release of CO2 1935Albert Szent-Gyorgyi showed that Succinate Fumarate Malate Oxaloacetate Carl Martius and Franz Knoop showed Citrate cis-aconitate isocitrate a ketoglutarate succinate fumarate malate oxaloacetate
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Martius and Knoop showed that pyruvate and oxaloacetate could form citrate non-enzymatically by the addition of peroxide under basic conditions. Krebs showed that succinate is formed from fumarate, malate or oxaloacetate. This is interesting since it was shown that the other way worked as well!! Pyruvate can form citrate enzymatically Pyruvate + oxaloacetate citrate + CO2 The interconversion rates of the intermediates was fast enough to support respiration rates.
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Overview
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The citric acid cycle enzymes are found in the matrix of the mitochondria Substrates have to flow across the outer and inner parts of the mitochondria
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Nathan Kaplan and Fritz Lipmann discovered Coenzyme A and Ochoa and Lynen showed that acetyl-CoA was intermediate from pyruvate to citrate.
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CoA acts as a carrier of acetyl groups Acetyl-CoA is a “high energy” compound: The DG' for the hydrolysis of its thioester is -31.5 kJ• mol-1 making it greater than the hydrolysis of ATP Pyruvate dehydrogenase converts pyruvate to acetyl-CoA and CO2
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Pyruvate dehydrogenase A multienzyme complexes are groups of non covalently associated enzymes that catalyze two or more sequential steps in a metabolic pathway. E. coli yeast Pyruvate dehydrogenase -- E1 24 60 dihydrolipoyl transacetylase --E2 24 60 dihydrolipoyl dehydrogenase--E3 12 12 Molecular weight of 4,600,000 Da
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24 E2 subunits 24 E1 orange a and b together 12 E3 Red
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EM based image of the core E2 from yeast pyruvate dh 60 subunits associated as 20 cone-shaped trimers that are verticies of a dodecahedron
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Why such a complex set of enzymes? 1 Enzymatic reactions rates are limited by diffusion, with shorter distance between subunits a enzyme can almost direct the substrate from one subunit (catalytic site) to another. 2. Channeling metabolic intermediates between successive enzymes minimizes side reactions 3. The reactions of a multienzyme complex can be coordinately controlled
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Covalent modification of eukaryotic pyruvate dehydrogenase
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The five reactions of the pyruvate dehydrogenase multi enzyme complex
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The enzyme requires five coenzymes and five reactions Pyruvate + CoA + NAD+ acetyl-CoA + CO2 + NADH
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The Coenzymes and prosthetic groups of pyruvate dehydrogenase Cofactor Location Function Thiamine Bound to E1 Decarboxylates pyrophosphate pyruvate Lipoic acid Covalently linked Accepts to a Lys on hydroxyethyl E2 (lipoamide) carbanion from TPP CoenzymeA Substrate for E2 Accepts acetyl group from lipoamide FAD (flavin) Bound to E3 reduced by lipoamide NADH Substrate for E3 reduced by FADH2
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Domain structure of dihydrolipoyl transacetylase E2
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1. Pyruvate dh decarboxylates pyruvate using a TPP cofactor forming hydroxyethyl-TPP. 2 The hydroxyethyl group is transferred to the oxidized lipoamide on E2 to form Acetyl dihydrolipoamide-E2 3 E2 catalyzes the transfer of the acetyl groups to CoA yielding acetyl-CoA and reduced dihydrolipoamide-E2 4 Dihydrolipoyl dh E3 reoxidizes dihydrolipoamide-E2 and itself becomes reduced as FADH2 is formed 5 Reduced E3 is reoxidized by NAD+ to form FAD and NADH The enzymes SH groups are reoxidized by the FAD and the electrons are transferred to NADH Pyruvate dehydrogenase
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+ +
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S S FAD + SH SH FAD SH SH FAD S S FADH2 S S FAD NAD+ NADH + H+ +
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Arsenite or organic arsenical compound inhibition
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