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Polymerase Chain Reaction PowerPoint Presentation

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Description : POLYMERASE CHAIN REACTION

Tags : PCR,polymerase chain,chain reaction

Published on : Nov 20, 2022 | Views : 2305 | Downloads : 3

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Slide 1-

POLYMERASE CHAIN REACTION
Slide 2-

Contents
Slide 3-

Polymerase Chain Reaction
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Slide 5-

Polymerase Chain Reaction
Slide 6-

PCR
Slide 7-

PCR Reaction Components
Slide 8-

PCR Reaction Components
Slide 9-

DNA Template
Slide 10-

Primers
Slide 11-

Four Normal Deoxynucleosides Triphosphate
Slide 12-

The standard PCR buffer contains:
Slide 13-

DNA Polymerase
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Tm= 2 C  X (number of A and T bases)+4 C  X (number of G and C bases).
Slide 16-

STANDARD PCR REACTION
Slide 17-

PCR
Slide 18-

Avoiding Contamination
Slide 19-

Sample Handling
Slide 20-

Laboratory Facilities
Slide 21-

Working with RNA
Slide 22-

Polymerase Chain Reaction
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Thermal Cycling Profile for Standard PCR
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Each cycle includes three successive steps:
Slide 26-

PCR
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Number of Cycles
Slide 29-

Gel Electrophoresis
Slide 30-

Gel Electrophoresis
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Gel Tray/ Loading
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PCR: Three Phases
Slide 35-

Polymerase Chain Reaction
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Variant of PCR
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Reverse Transcriptase-PCR
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RT-PCR
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Nested PCR
Slide 40-

Nested-PCR
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Hot-Start PCR
Slide 42-

Hot-Start PCR
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What is Wrong with Agarose Gels?
Slide 44-

Real Time PCR
Slide 45-

Real Time PCR
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Polymerase Chain Reaction: Uses
Slide 47-

Infectious Diseases/ Cancer
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Genetic Disease
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Inherited disorders diagnosed using PCR protocols
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Prenatal Diagnosis
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Research
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References & Further Reading
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