Download Free Medical DIAGNOSTIC CYTOPATHOLOGY PowerPoint Presentation

DIAGNOSTIC CYTOPATHOLOGY PowerPoint Presentation

DOWNLOAD MEDICAL POWERPOINT PRESENTATION

About This Presentation

Description : Check out this medical presentation on Pathology, which is titled "DIAGNOSTIC CYTOPATHOLOGY", to know about diagnostic cytopathology and its significance in biopsy investigation.

Tags : Pathology, Diagnostic Cytopathology, Significance, Biopsy Investigation

Published on : Aug 03, 2021 | Views : 5547 | Downloads : 15

Shareable Url :

Embed Code :

Comment

Transcript

Slide 1-

DIAGNOSTIC CYTOPATHOLOGY DR. SANJIV KUMAR ASSTT. PROFESSOR, DEPTT. OF PATHOLOGY, BVC, PATNA
Slide 2-

CONTENTS INTRODUCTION APPLICATIONS TECHNIQUES SMEAR FORMATION FIXATION STAINING METHODS INTERPRETATIONS
Slide 3-

INTRODUCTION Is a branch of pathology that studies and diagnoses diseases on the cellular level. OR Interpretation of cells that has either exfoliated spontaneously or are obtained from various organs/ tissue by different clinical methods.
Slide 4-

PRINCIPLES OF CYTOTECHNIQUE TO REDUCE THE SPECIMEN TO A CELLULAR PRESENTATION, WHICH CAN BE INTERPRETED AND DIAGNOSED
Slide 5-

MATERIALS REQUIRED
Slide 6-

aDVANTAGES Simple Rapid test Inexpensive No or minimum injury during sampling DISADVANTAGES Interpretation based on few cells It is not a final diagnosis Cannot determine size and type of lesions on some cases
Slide 7-

Remember CYTOPATHOLOGY SHOULD BE COMPARED TO HISTOPATHOLOGY!
Slide 8-

APPLICATIONS Cytogenetics-for chromosomal studies. Identification of benign neoplasms Diagnosis and management of cancer. Diagnosis of non-neoplastic/inflammatory conditions. Diagnosis of specific infections. Hormonal imbalance. To follow up progress or improvement in disease process. Diagnosis of specific infections. A variety of bacterial, viral, protozoal and fungal infections can be identified by cytological methods.
Slide 9-

STEPS IN CYTOPATHOLOGY SAMPLE COLLECTION SMEAR PREPARATION FIXATION OF SAMPLE STAINING OF SMEAR INTERPRETATION
Slide 10-

SAMPLES Exfoliated cells, Aspirated cells and body fluids The sample can be collected by: scrapping the lateral walls of vagina fresh early morning sputum bronchial washings buccal smears gastric lavage fresh catheterized urine samples pericardial, pleural and peritoneal fluids CSF Etc.
Slide 11-

Preparation of samples
Slide 12-

TECNIQUES OF CYTOPATHOLOGY EXFOLIATIVE CYTOLOGY This is an older branch that essentially involves the study of cells spontaneously shed off from epithelial surfaces into body cavity or body fluids. Basis is alteration in morphology and less cohesiveness of cells. INTERVENTIONAL CYTOLOGY This is the branch in which samples are obtained by clinical procedures or surgical intervention. It is also known as aspiration cytology.
Slide 13-

BLOOD LESS TYPE
Slide 14-

INTERVENTIONAL CYTOLOGY This is the branch in which samples are obtained by clinical procedures or surgical intervention. It is also known as aspiration cytology. It includes fine needle aspiration cytology (FNAC), Biopsy, imprint cytology and crush smear cytology.
Slide 15-

IMPRINT CYTOLOGY: here touch preparations from cut surfaces of superficial excised lesions are prepared. Advantage is that the cell distribution reflects the tissue architecture thus aiding in interpretation. ii. CRUSH SMEAR CYTOLOGY: are helpful in diagnosis of tumors. It provides the recognition of tissue architecture in addition to better cytological details. iii. FNAC: It has gained importance in the last three decades. Almost all fine needle accessible organs can be aspirated for cytological studies. iv. BIOPSY: Removal of tissue from a living animal for examination (microscopic & chemical) so that a diagnosis can be made.
Slide 16-

Disadvantages: Chances of haematomas, infection, Pneumothorax etc. Involves surgical interaction. Collect fewer cells than scrapings.
Slide 17-

IMRINT CYTOLOGY
Slide 18-

CRUSH SMEAR
Slide 19-

FNAC APPLICATIONS Applied in diagnosis of Palpable as well as Non palpable lesion PALPABLE MASS LESION i)Lymph node ii)Breast (duct carcinoma) iii)Thyroid iv)Salivary gland v) Soft tissue masses vi)Bones NON PALPABLE MASS LESION i)Abdominal cavity ii)Thoracic cavity iii)Retroperitonium
Slide 20-

Taking palpable mass in FNAC-
Slide 21-

LIMITATIONS
Slide 22-

ppt slide no 22 content not found
Slide 23-

ppt slide no 23 content not found
Slide 24-

ppt slide no 24 content not found
Slide 25-

ppt slide no 25 content not found
Slide 26-

ppt slide no 26 content not found
Slide 27-

ppt slide no 27 content not found
Slide 28-

ppt slide no 28 content not found
Slide 29-

ppt slide no 29 content not found
Slide 30-

ppt slide no 30 content not found
Slide 31-

ppt slide no 31 content not found
Slide 32-

FIXATION
Slide 33-

ppt slide no 33 content not found
Slide 34-

DIAGNOSTIC CYTOPATHOLOGY DR. SANJIV KUMAR ASSTT. PROFESSOR, DEPTT. OF PATHOLOGY, BVC, PATNA
Slide 35-

All material for cytological examination must be properly fixed to ensure preservation of cytomorphological details. Methods of fixing vary depending upon the type of staining employed. Materials is usually wet-fixed for use with Papanicolaou or H&E staining. Sometimes, air-dried for use in Romanowsky staining. Special purpose fixative may be buffered neutral formalin, bouin’s fluid and picric acid
Slide 36-

ppt slide no 36 content not found
Slide 37-

STAINING WET FIXED i. Papanicolaou or H&E staining. AIR DRY FIXED Romanowsky staining. Wright’s stain. Giemsa stain. May Grunwald giemsa stain. Wright’s Giemsa stain.
Slide 38-

papanicolaou staining wet fixed
Slide 39-

ppt slide no 39 content not found
Slide 40-

ppt slide no 40 content not found
Slide 41-

INTERPRETATION NORMAL ABNORMAL INFLAMMATORY NON INFLAMMATORY NON SEPTIC SEPTIC NON NEOPLASTIC NEOPLASTIC HYPERTROPHY MALIGNANT BENIGN HYPERPLASIA
Slide 42-

ppt slide no 42 content not found
Slide 43-

BEAUTY OF CYTOPATHOLOGY Guess the images ?????
Slide 44-

ppt slide no 44 content not found
Slide 45-

ppt slide no 45 content not found
Slide 46-

ppt slide no 46 content not found
Slide 47-

ppt slide no 47 content not found
Slide 48-

ppt slide no 48 content not found
Slide 49-

ppt slide no 49 content not found
Slide 50-

ppt slide no 50 content not found
Slide 51-

ppt slide no 51 content not found