Download Free Medical Dental Plaque PowerPoint Presentation

Dental Plaque PowerPoint Presentation

DOWNLOAD MEDICAL POWERPOINT PRESENTATION

About This Presentation

Description : Check out this medical presentation on Dental Calculus, which is titled "Dental Plaque", to know about the difference between dental plaque, calculus , and materia alba, types of plaque, composition of plaque, and formation of plaque.

Tags : Dental Calculus, Dental Plaque, materia alba

Published on : Jul 01, 2021 | Views : 4515 | Downloads : 42

Shareable Url :

Embed Code :

Comment

Transcript

Slide 1-

DENTAL PLAQUE
Slide 2-

Most important chapter Understanding required Health maintenance Disease progression
Slide 3-

OBJECTIVES DEFINITION Difference between plaque, calculus , materia alba Types of plaque Composition of plaque Formation of plaque
Slide 4-

Lecture 2 Microscopic structure of plaque Concept of biofilm Plaque Hypothesis Microbial complexes Microbial tests for plaque sample
Slide 5-

Definition Dental Plaque “is a specific but highly variable structural entity, resulting from sequential colonization of microorganisms on tooth surfaces, restorations & other parts of oral cavity, composed of salivary components like mucin, desquamated epithelial cells, debris & microorganisms, all embedded in extracellular gelatinous matrix.” W.H.O ,1961
Slide 6-

Dental Plaque can be defined as: “ soft deposits that form the biofilm adhering to the tooth surface or other hard surfaces in the oral cavity, including removable & fixed restorations” Bowen , 1976
Slide 7-

DIFFERENTIATION:
Slide 8-

Materia Alba “soft accumulations of bacteria and tissue cells; lack organised structure of dental plaque; easily displaced by water spray” Dental Calculus ‘mineralised dental plaque”
Slide 9-

DETECTION OF DENTAL PLAQUE DETECTION Visual Periodontal Probe or Explorer Disclosing Agents
Slide 10-

Timeline of plaque development: At birth: sterile Hours: facultative aerobic bacteria Second day: anaerobic bacteria 2 weeks: mature microbiota Weaning (> 2 years): 400 different types of bacteria Body contains 10 times more bacteria than human cells
Slide 11-

Open growth system: communication with the pharynx > 500 species in mouth In any individual: 150 or more species at any given time.
Slide 12-

Mastication Tongue Oral hygiene Washout effect: saliva Ciliae Soft tissue Hard tissue
Slide 13-

Niches of plaque accumulation: Supragingival and hard surgfaces: teeth, implant, restorations. Periodontal pocket (hard: root cementum & soft: pocket epithelium) Buccal, palatal and floor of the mouth epithelium Dorsum of the tongue Tonsils
Slide 14-

Natural cleansing mechanism: Gingival crevicular fluid & salivary flow Cleansing effect of mastication and tongue movement Rapid turnover rate of intraoral epithelial cells Host defence mechanisms like langerhans cells.
Slide 15-

Hard tissues Teeth and implants surface provide a non shedding surface. It allows extensive plaque accumulation. Provide an ectodermal interruption. At junctional epithelium the teeth provide access to bacteria into the body. Port of entry of periopathogens.
Slide 16-

Macroscopic Structure Supragingival Plaque Subgingival Plaque Marginal Plaque
Slide 17-

Composition of Plaque Microorganisms Intercellular Matrix
Slide 18-

Microorganisms Bacterial 1gm contains 2X1011 bacteria-500 species Non-Bacterial Mycoplasma, Yeast, Protozoa, Viruses
Slide 19-

Intercellular Matrix Accounts for 20% to 30% of the plaque mass Organic and inorganic materials Derived from saliva, gingival crevicular fluid, and bacterial products.
Slide 20-

Organic Component Organic constituents : polysaccharides, proteins, glycoproteins, and lipid material Polysaccharides produced by bacteria-Dextran: predominant form Albumin: originating from crevicular fluid Lipid material: debris from the membranes of disrupted bacterial and host cells and possibly food debris.
Slide 21-

Inorganic Component Calcium and phosphorus (Most) Trace amounts: sodium, potassium and fluoride. Source -supragingival plaque (saliva) & subgingival plaque (GCF & Blood) Calculus frequently found in areas of the dentition adjacent to salivary ducts
Slide 22-

PHASES OF PLAQUE FORMATION
Slide 23-

Formation of dental Pellicle Initial Colonization Secondary Colonization & Plaque Maturation
Slide 24-

Formation of dental Pellicle Acquired enamel pellicle forms rapidly - Early pellicle Characterized by an absence of bacteria and their products. Composed of proteins and glycoproteins. Demonstrate a higher content of threonine, serine, and alanine & less proline than saliva- selective adsorption
Slide 25-

Electron microscopic -reveals a thin, amorphous, electron-dense layer immediately adjacent to the hard surface- thickness from 1 to 2 nm. involves a combination of physical forces (ionic, hydrophobic, hydrogen bonding, and van der Waals)
Slide 26-

May involve the interaction of phosphate groups with calcium ions in saliva to form "bridges" Protective functions of early enamel pellicle: protection , lubrication by decreasing frictional forces, may selectively concentrate antimicrobial substances such as immunoglobulins, lysozyme, and cystatins at different oral surfaces.
Slide 27-

Formation of later pellicle most likely involves protein-protein or protein-carbohydrate interactions,- stereospecific in nature . For example, A. viscosus and Streptococcus mitis produce a neuraminidase that cleaves terminal sialic acid residues on the glycoproteins in saliva or early pellicles to expose galactose residues (Costello et aI., 1979) Collectively, these mechanisms may be important for the initial colonization
Slide 28-

Initial adhesion Phase I : Transport to the surface Phase II : Initial adhesion Phase III : Attachment Phase IV : Colonisation of the surface and biofilm formation
Slide 29-

ppt slide no 29 content not found
Slide 30-

ppt slide no 30 content not found
Slide 31-

Phase I Random contacts: Brownian motion Sedimentation of micro- organisms Liquid flow Active bacterial movement
Slide 32-

Phase II Initial reversible adhesion Long range and short range forces: van der Walls attractive forces Electrostatic repulsive forces
Slide 33-

Phase III Firm anchorage Specific interactions: Covalent Ionic Hydrogen bonding
Slide 34-

ppt slide no 34 content not found
Slide 35-

Adhesions: specific extracellular proteinacious components on microorganisms. Complimentary receptors: proteins, glycoproteins or polysaccharides on the pellicle surface.
Slide 36-

Example: S. Sanguis- binds to acidic proline rich proteins, alpha amylase & sialic acid A. viscosus- fimbriae that contain adhesins- bind to proline rich proteins of dental pellicle.
Slide 37-

Colonisation & Plaque maturation Initial colonisers attach to the tooth surface- provide substrate for secondary colonisers to attach. They create a favourable environment for secondary colonisers to survive.
Slide 38-

Secondary Colonisers: Adhere to bacteria already present in the plaque mass. Prevotella intermedia Prevotella loeschii Fusobacterium nucleatum Porphyromonas gingivalis Capnocytophaga
Slide 39-

Co- aggregation Cell to cell recognition of genetically distinct partner cell types Highly specific stereochemical interaction Corn cob formation: long filament bacteria covered with cocci Test tube brush: long filament bacteria covered with flagellated small motile rods
Slide 40-

MICROSCOPIC STRUCTURE
Slide 41-

MICROSCOPIC STRUCTURE SUPRAGINGIVAL PLAQUE typically demonstrates a stratified organization of the bacterial morphotypes. Gram-positive cocci and short rods predominate at the tooth surface gram-negative rods and filaments ,spirochetes predominate in the outer surface of the mature plaque mass. Highly specific cell-to-cell interactions are also evident from the "corncob"
Slide 42-

CORN-COB STRUCTURE CORN COB STRUCTURE
Slide 43-

Thin section of supragingival plaque GRAM POSITIVE BACTERIA IN PALISADING ARRANGEMENT
Slide 44-

SUBGINGIVAL PLAQUE Gingival crevicular fluid, -contains many substances that the bacteria may use as nutrients Host inflammatory cells and mediators have influence on the establishment and growth of bacteria in this region. Distinctions present between tooth-associated and tissue-associated regions of subgingival plaque
Slide 45-

ppt slide no 45 content not found
Slide 46-

Thin section of plaque in a deep pocket FILAMENTS RODS COCCI
Slide 47-

DENTAL PLAQUE AS BIOFILM
Slide 48-

Plaque-A Biofilm 1.Structure-microcolonies of bacteria in matrix 2.Exopolysaccharides produced by bacteria 50-90% of dry wt integrity of biofilm buffer
Slide 49-

3.Physiological Heterogenicity Same sp.-different physiologic states in biofilms 4.Quorum Sensing “regulation of expression of specific genes”- intercellular communication distinct properties Prosser,1999 5.Attachment of Bacteria- fimbrae & fibrils
Slide 50-

6.Increased antibiotic Resistance Bacteria in biofilms more resistant Slow growth Decreased diffusion Accumulation of enzymes- lactamases,dehydrogenases Alteration of genes.
Slide 51-

METABOLIC INTERACTIONS
Slide 52-

Microbial Complexes microbiota into groups or complexes, - appear to occur together transition from a healthy oral environment to gingivitis and to periodontal disease is triggered by a specific 'set' or 'complex' of bacterial species Socransky and Haffajee and colleagues,1998
Slide 53-

THE VARIOUS COMPLEXES
Slide 54-

Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the 'red complex‘ Several characteristics make them prime candidates as pathogens in the clinical destruction of periodontal tissues: 1. occur concomitantly with the clinical signs of periodontal destruction; 2.appear closely 'linked' topologically in the developing biofilm; 3. in vitro studies demonstrate their ability to produce a number of outer membrane-associated proteinases
Slide 55-

Association of Plaque microorganisms with Periodontal diseases Early 1900-papers on specific organisms Mid 1930s-”bacteria-dental orphans” Belding & Belding 1936
Slide 56-

“Non Specific Plaque Hypothesis” Walter Loeche, 1976 “Specific Plaque Hypothesis” Walter Loeche , 1976 A.actinomycetemcomitans-LAgP Newman,Socransky,1977 “Specific Plaque Hypothesis revisited” Specific strains
Slide 57-

MICROORGANISMS IN SPECIFIC PERIODONTAL DISEASES
Slide 58-

Total no. of bacteria per gram of plaque-twice in periodontally diseased sites Healthy sites-gram +ve facultative rods & cocci J Slots ,1977 Protective species-S.Sanguis,C.Ochracea Dzink JL ,1985
Slide 59-

Plaque induced Gingivitis- Harold Loe, 1965 Gram positive - S.Sanguis,S. intermedius,S.Oralis,A. viscosus etc Gram negative-F.nucleatum,P.intermedia,Capnocytophaga Pregnancy induced gingivitis P. intermedia
Slide 60-

Chronic periodontitis Spirochetes, gingivalis, B.forsythus, A.actinomycetemcomitans P. micros, Treponema
Slide 61-

Localized Aggressive Periodontitis – A.actinomycetemcomitans Socransky, 1977 P. gingivalis, F. nucleatum
Slide 62-

Necrotizing Periodontal diseases P. intermedia Abcesses of Periodontium F.nucleatum P. intermedia, P. gingivalis, P. micros
Slide 63-

CRITERIA for ASSOCIATION of PERIODONTAL PATHOGENS
Slide 64-

KOCH’S POSTULATES In I870s, Robert Koch’s criteria for causative agent in human infections. 1. Be routinely isolated from diseased individuals 2. Be grown in pure culture in the laboratory 3. Produce a similar disease when inoculated into susceptible laboratory animals 4. Be recovered from lesions in a diseased laboratory animal
Slide 65-

SIGMUND SOCRANSKY’S CRITERIA FOR PERIODONTAL PATHOGENS 1. Be associated with disease, as evident by increases in the number of organisms at diseased sites 2. Be eliminated or decreased in sites that demonstrate clinical resolution of disease with treatment 3. Demonstrate a host response, in the form of an alteration in the host cellular or humoral immune response 4. Be capable of causing disease in experimental animal models 5. Demonstrate virulence factors responsible for enabling the microorganism to cause destruction of the periodontal.tissues
Slide 66-

EVIDENCE SUPPORTING ROLE OF MICRORGANISMS AS PATHOGENS IN PERIODONTAL DISEASE; SOCRANSKY’S CRITERIA
Slide 67-

ppt slide no 67 content not found
Slide 68-

MICROBIOLOGICAL TESTS FOR PLAQUE SAMPLES Microscopic identification Microbiological culturing Enzymatic assays Immunoassays Nucleic acid probes Polymerase chain reaction assays
Slide 69-

MICROSCOPIC IDENTIFICATION Light Microscope Scanning Electron Microscope Transmission Electron Microscope
Slide 70-

MICROBIOLOGICAL CULTURING Culture methods -the gold standard Available for the positive identification of many putative periodontopathogenic microorganisms Use of selective and non-selective media One unique advantage - permits the assessment of antibiotic sensitivity. Disadvantages- inability to detect low levels of microorganisms, high cost, labor intensiveness, prolonged time ,difficulty in growing several bacterial species
Slide 71-

IMMUNOASSAYS Immunofluorescence microscopy, enzyme-linked immunosorbent assay (ELISA), membrane assays and latex agglutination assays. Advantages- include fairly low detection thresholds, relatively low cost, rapid, are somewhat quantitative Disadvantages- do not permit evaluation of antibiotic sensitivity of the flora.
Slide 72-

ELISA Partially purified, inactivated antigens pre-coated onto an ELISA plate Patient serum which may contain antibodies. Anti-human immunoglobulin coupled to an enzyme. -second antibody, -binds to human antibodies Chromogen or substrate which changes color when cleaved by the enzyme attached to the second antibody.
Slide 73-

ELISA Negative ELISA Test Positive ELISA Test
Slide 74-

NUCLEIC ACID PROBES Consist of nucleic acid sequences labeled with a radioactive or enzymatic-colorimetric marker Bind to complementary nucleic acid sequences on corresponding microorganisms. Newer-synthetic oligonucleotides (also known as 16s rRNA probes) Oligonucleotide probes have greatest specificity and lowest cross-reactivity as they target genes specific to a bacterial species. Advantages-greater sensitivity than culture methods,viability of microorganisms is not a requirement
Slide 75-

POLYMERASE CHAIN REACTION A molecular biological technique for high-yield replication of DNA. Allows to synthesize vast numbers of copies of minute samples of DNA Modification of the original PCR technology, "real-time" PCR, permits detection of specific microorganisms & also its quantification PCR assays, used in combination with synthesized 16S rRNA probes -enable the detection of virtually any microorganism in a plaque sample
Slide 76-

ENZYMATIC ASSAYS Enzymatic assay - detects bacteria that possess trypsin-like enzymes such as T. Forsythensis, treponema denticola and P. Gingivalis. Plaque sample containing any combination of these three bacteria- placed on a paper strip impregnated with a colorless substrate n-benzoyl-dl-arginine-2-naphthylamide (BANA) BANA substrate breakdown produces a blue-black color Disavantages-test unable to distinguish between relative proportions of the three bacteria,cannot identify the presence of other oral microorganisms,.