Download Free Medical Amyloidosis PowerPoint Presentation

Amyloidosis PowerPoint Presentation

DOWNLOAD MEDICAL POWERPOINT PRESENTATION

About This Presentation

Description : Check out this medical presentation on amyloidosis, which is titled "Amyloidosis", to know about amyloid as an abnormal proteinaceous substance that is deposited between cells in many tissues and organs of the body in a variety of clinical disorders.

Tags : amyloidosis, amyloid, proteinaceous substance, cells, tissues, clinical disorders

Published on : May 06, 2021 | Views : 5437 | Downloads : 4

Shareable Url :

Embed Code :

Comment

Transcript

Slide 1-

1
Slide 2-

2 AMYLOIDOSIS DEFINITION- AMYLOID IS AN ABNORMAL PROTEINACEOUS SUBSTANCE THAT IS DEPOSITED BETWEEN CELLS IN MANY TISSUES & ORGANS OF THE BODY IN A VARIETY OF CLINICAL DISORDERS.
Slide 3-

3 AMYLOIDOSIS AMYLOID APPEARS AS AN INTERCELLULAR PINK TRANSLUCENT MATERIAL
Slide 4-

4 AMYLOIDOSIS WORD MEANING IS -STARCH LIKE. IT IS A GROUP OF DISEASES THAT SHARE IN COMMON THE DEPOSITION OF SIMILAR APPEARING PROTEINS.
Slide 5-

5 PHYSICAL NATURE OF AMYLOID E M – 1 .PROTEIN COMPONENT(95%) - NONBRANCHING FIBRILS OF WIDTH 7.5 -10 nm X RAY CRYSTALLOGRAPHY, INFRARED SPECTROSCOPY-CROSSED β PLEATED SHEET CONFORMATION SECOND COMPONENT – ( 5%) MINOR NONFIBRILLAR PENTAGONAL P COMPONENT-A GLYCOPROTEIN
Slide 6-

PHYSICAL NATURE OF AMYLOID 2.P COMPONENT & OTHER GLYCOPROTEINS - 5% OF AMYLOID – NONFIBRILLAR PENTAGONAL 6
Slide 7-

7 AMYLOID PROTEIN-NONBRANCHING FIBRILS HAVING CROSSED β PLEATED PATTERN
Slide 8-

8 CHEMICAL NATURE OF AMYLOID 2 MAJOR CHEMICAL CLASSES 1. IMMUNOGLOBULIN LIGHT CHAIN AL (AMYLOID LIGHT CHAIN)- IS MADE UP OF COMPLETE Ig LIGHT CHAIN
Slide 9-

9 CHEMICAL NATURE OF AMYLOID OR N TERMINAL FRAGMENT OF LIGHT CHAINS OR BOTH. MOST FREQUENTLY AL IS LAMDA LIGHT CHAIN 2 . NONIMMUNOGLOBULIN PROTEIN- AA (AMYLOID ASSOCIATED PROTEIN)
Slide 10-

10 CHEMICAL NATURE AL AMYLOID PROTEIN IS ASSOCIATED WITH B- CELL DYSCRASIAS & IS PRODUCED BY IMMUNOGLOBULIN SECRETING CELLS.
Slide 11-

11 CHEMICAL NATURE AA IS A NONIMMUNOGLOBULIN PROTEIN WITH MOLECULAR WEIGHT 8500 DALTONS DERIVED FROM PRECURSOR PROTEIN SAA WHICH IS AN ACUTE PHASE REACTANT.
Slide 12-

12
Slide 13-

13 OTHER PROTEINS FOUND IN AMYLOID 1 . TRANSTHYRETIN- NORMAL SERUM PROTEIN – TRANSPORTS THYROXIN & RETINOL- MUTANT FORM OF TRANSTHYRETIN – AMY LOID TRANSTHYRETIN (ATTR) IS DEPOSITED IN A GROUP OF GENETIC DISORDERS KNOWN AS FAMILIAL AMYLOID POLYNEUROPATHIES.
Slide 14-

14 OTHER PROTEINS FOUND IN AMYLOID TRANSTHYRETIN IS DEPOSITED AS AMYLOID WITH AGING IN THE HEART.AMYLOID IN SUCH CASES IS STRUCTURALLY NORMAL(SENILE SYSTEMIC AMYLOIDOSIS)
Slide 15-

15 OTHER PROTEINS FOUND IN AMYLOID 2 .β 2 MICROGLOBULIN- A COMPONENT OF MHC CLASS 1 MOLECULE- A NORMAL SERUM PROTEIN- AS AMYLOID FIBRIL SUBUNIT (Aβ2m) ( INCREASED IN RENAL DISEASES) SEEN IN AMYLOIDOSIS ASSOCIATED WITH LONG TERM HAEMODIALYSIS.
Slide 16-

16 CHEMICAL NATURE 3 . β AMYLOID PROTEIN( Aβ ) - A 4kD PEPTIDE SEEN IN CEREBRAL PLAQUE & WALLS OF CEREBRAL BLOOD VESSELS IN ALZHEIMER DISEASE- DERIVED FROM AMYLOID PRECURSOR PROTEIN (APP).
Slide 17-

17 CHEMICAL NATURE 4. HORMONE PRECURSORS (PROCALCITONIN) & KERATIN
Slide 18-

18 CLASSIFICATION OF AMYLOIDOSIS 1. SYSTEMIC 2.LOCALISED SYSTEMIC IS SUBCLASSIFIED INTO A.PRIMARY B.SECONDARY AMYLOIDOSIS C. HEREDITARY OR FAMILIAL AMYLOIDOSIS
Slide 19-

CLASSIFICATION OF AMYLOIDOSIS a. FAMILIAL MEDITERANEAN FEVER b.FAMILIAL AMYLOIDOTIC NEUROPATHIES(SEVERAL TYPES) c.SYSTEMIC SENILE AMYLOIDOSIS 19
Slide 20-

CLASSIFICATION OF AMYLOIDOSIS D.HAEMODIALYSIS – ASSOCIATED AMYLOIDOSIS 20
Slide 21-

CLASSIFICATION OF AMYLOIDOSIS LOCALIZED AMYLOIDOSIS A. SENILE CEREBRAL –ALZHEIMER DISEASE B.ENDOCRINE a. MEDULLARY CARCINOMA THYROID b. ISLET OF LANGERHANS IN TYPE 2 DIABETES 21
Slide 22-

22 PRIMARY SYSTEMIC AMYLOIDOSISIMMUNOCYTE DYSCRASIAS WITH AMYLOIDOSIS MOST COMMON – 1.MULTIPLE MYELOMA- MALIGNANT B CELLS SECRETE MYELOMA PROTEIN(M PROTEIN) & LAMDA & KAPPA LIGHT CHAINS (BENCE JONES PROTEINS –SERUM & URINE).
Slide 23-

23 PRIMARY AMYLOIDOSIS(IMMUNOCYTE DYSCRASIAS WITH AMYLOIDOSIS) 6 – 15 % OF MYELOMA PATIENTS WHO HAVE FREE LIGHT CHAINS DEVELOP AMYLOIDOSIS.
Slide 24-

24 PRIMARY AMYLOIDOSIS 2. OTHER β CELL DYSCRASIAS WITHOUT TUMOUR MASSES
Slide 25-

25 SECONDARY AMYLOIDOSIS(REACTIVE SYSTEMIC AMYLOIDOSIS) 1. PROTRACTED CELL INJURY DUE TO INFECTIOUS & NONINFECTIOUS CHRONIC INFLAMMATORY CONDITIONS. 2. TUMOURS LIKE RENAL CELL CARCINOMA, HODGKIN DISEASE.
Slide 26-

26 HEREDOFAMILIAL AMYLOIDOSIS RARE, OCCUR IN LIMITED GEOGRAPHIC AREAS, MEDITARRANEAN FEVER- AUTOSOMAL RECESSIVE FEVER WITH SEROSAL INFLAMMATION- IN ARMANIAN SEPHERDIC JEWISH & ARABIC ORIGINS- AMYLOID DEPOSITED IS AA PROTEIN
Slide 27-

27 HEREDOFAMILIAL AMYLOIDOSIS 2 . FAMILIAL AMYLOIDOTIC POLYNEUROPATHIES AUTOSOMAL DOMINANT. AMYLOID DEPOSITION IN PERIPHERAL & AUTONOMIC NERVES PORTUGAL, JAPAN ,SWEDEN & U S – MUTANT TRANSTHYRETINS(ATTR)
Slide 28-

28 LOCALISED AMYLOIDOSIS SINGLE ORGAN OR TISSUE MAY PRODUCE NODULAR MASSES OR MAY BE MICROSCOPIC
Slide 29-

29 LOCALISED AMYLOIDOSIS LUNG, LARYNX,SKIN ,URINARY BLADDER,TONGUE & ABOUT THE EYE LYMPHOCYTES & PLASMA CELLS ARE SEEN ABOUT THE MASSES. IN SOME CASES AMYLOID CONSIST OF AL PROTEIN
Slide 30-

30 ENDOCRINE AMYLOID 1. MEDULLARY CARCINOMA OF THYROID 2. ISLET TUMOURS OF PANCREAS, 3. PHEOCHROMOCYTOMA 4. UNDIFFERENTIATED CARCINOMA OF STOMACH
Slide 31-

31 ENDOCRINE AMYLOID 5. ISLETS OF LANGERHANS IN TYPE 2 DIABETES IN THESE SETTINGS AMYLOIDOGENIC PROTEINS ARE DERIVED FROM POLYPEPTIDE HORMONES OR ISLET AMYLOID POLYPEPTIDE (IAPP)
Slide 32-

32 AMYLOID OF AGING SENILE SYSTEMIC AMYLOIDOSIS 70 -80 YEAR OLD.PRESENT AS RESTRICTIVE CARDIOMYOPATHY HENCE THE NAME SENILE CARDIAC AMYLOIDOSIS – TRANSTHYRETIN & MUTANT FORM OF TRANSTHYRETIN
Slide 33-

33 REACTIVE SYSTEMIC AMYLOIDOSIS LONG STANDING TISSUE INJURY & INFLAMMATION- ELEVATED LEVELS OF SAA PROTEIN SYNTHESIZED BY THE LIVER UNDER THE INFLUENCE OF CYTOKINES –IL-1 &IL- 6.
Slide 34-

34 REACTIVE SYSTEMIC AMYLOIDOSIS SAA IS DEGRADED TO SOLUBLE END PRODUCTS BY THE ACTION OF MONOCYTE –DERIVED ENZYMES
Slide 35-

35 PATHOGENESIS IN INDIVIDUALS WITH ENZYME DEFECT – INCOMPLETE BREAKDOWN OF SAA- INSOLUBLE AA MOLECULES PRIMARY AMYLOIDOSIS- AMYLOID MATERIAL IS DERIVED FROM DEFECTIVE PROTEOLYSIS OF IMMUNOGLOBULIN LIGHT CHAINS
Slide 36-

36 PATHOGENESIS ALZHEIMER DISEASE- BREAKDOWN PRODUCT OF AMYLOID PRECURSOR PROTEIN (APP) CODED BY LOCUS ON 21 CHROMOSOME. BREAKDOWN PRODUCT OF APP IS β AMYLOID(Aβ)
Slide 37-

37 MORPHOLOGY ORGAN DISTRIBUTION IS DIFFERENT IN DIFFERENT TYPES SECONDARY AMYLOIDOSIS- KIDNEY ,LIVER, SPLEEN,LYMPH NODES, ADRENALS, THYROID & MANY OTHER TISSUES.
Slide 38-

38 MORPHOLOGY IMMUNOCYTE ASSOCIATED AMYLOIDOSIS- THE ORGANS INVOLVED ARE HEART, GIT,R S, PERIPHERAL NERVES, SKIN ,TONGUE
Slide 39-

39 MORPHOLOGY CONTI- HEREDOFAMILIAL AMYLODOSIS – DISTRIBUTION MAY BE WIDE SPREAD OR LOCALISED
Slide 40-

40 MORPHOLOGY CONTI- AMYLOID DEPOSITS MAY OR MAY NOT BE APPARENT GROSSLY SMALL AMOUNTS CAN BE RECOGNISED BY PAINTING THE CUT SURFACE WITH IODINE & SULFURIC ACID- MAHOGANY BROWN COLOUR
Slide 41-

41 MORPHOLOGY WHEN LARGE AMOUNT IS DEPOSITED- ORGAN IS ENLARGED- TISSUE APPEARS GREY WITH A WAXY FIRM CONSISTANCY
Slide 42-

42 MORPHOLOGY MICROSCOPY – DEPOSITION IS BETWEEN THE CELLS OFTEN CLOSELY ADJACENT TO THE BASEMENT MEMBRANE. IN COURSE OF TIME AMYLOID ENCROACHES ON THE CELLS- ATROPHIC AND DISAPPEAR
Slide 43-

43 MORPHOLOGY IN IMMUNOCYTE -ASSOCIATED AMYLOIDOSIS - PERIVASCULAR & VASCULAR LOCALISATION IS COMMON.
Slide 44-

44 MORPHOLOGY THE HISTOLOGIC DIAGNOSIS OF AMYLOIDOSIS IS BASED ENTIRELY ON ITS STAINING CHARACTERISTICS
Slide 45-

45 STAINING OF AMYLOID GROSS STAINING- SLICE OF THE TISSUE IS DIPPED IN LUGOL’S IODINE FOLLOWED BY DIPPING IN DILUTE SULFURIC ACID – AMYLOID GIVES DARK BLUE COLOUR WITH IODINE & IT BECOMES BROWN WITH DILUTE SULFURIC ACID.
Slide 46-

46 MICROSCOPIC STAINING 1.H & E- AMYLOID IS PINK,EOSINOPHILIC 2.PAS – PINK COLOUR
Slide 47-

47 MICROSCOPIC STAINING 3. WITH METACHROMATIC STAINS – A COLOUR MAY EMERGE THAT IS NOT VISIBLE IN THE ORIGINAL DYE OR IN IT’S COMBINATION – THE PROPERTY IS KNOWN AS METACHROMASIA & SUCH DYES ARE KNOWN AS METACHROMATIC DYES
Slide 48-

48 METACHROMATIC STAINS THE TWO MOST COMMONLY USED METACHROMATIC DYES ARE A. . METHYL VIOLET – AMYLOID GIVES BRIGHT PINK COLOUR B. CRYSTAL VIOLET –BRIGHT PINK COLOUR
Slide 49-

49 MICROSCOPIC STAINING 4. OTHER STAINS ARE CONGO RED- PINK COLOUR- WHEN SEEN UNDER POLARIZING MICROSCOPE - AMYLOID GIVES APPLE GREEN BIREFRINGENCE (MOST DEPENDABLE STAIN)
Slide 50-

50 CONGO RED ,CONGO RED WITH POLARIZING MICROSCOPY-APPLE GREEN BIREFRINGENCE
Slide 51-

51 STAINING OF AMYLOID 5.FLUORESCENT STAINS- A.THIOFLAVIN T- YELLOW FLUORESCENCE, B THIOFLAVIN S – RED FLUORESCENCE,
Slide 52-

52 OTHER METHODS OF IDENTIFICATION ULTRASTRUCTURE OF AMYLOID- 1. E M - FIBRILLAR STRUCTURE 2. X RAY CRYSTALOGRAPHY & INFRARED SPECTROSCOPY- β PLEATED APPEARANCE.
Slide 53-

53 OTHER METHODS OF IDENTIFICATION 3. IMMUNOHISTOCHEMISTRY- PROTEIN COMPONENT CAN BE IDENTIFIED BY MONOCLONAL ANTI BODIES.
Slide 54-

54 MORPHOLOGY 1.KIDNEY- SIZE – NORMAL OR ENLARGED- PALE GREY & FIRM, IN LONG STANDING CASES KIDNEY IS SMALL
Slide 55-

55 MORPHOLOGY MICROSCOPY – DEPOSITS ARE FOUND IN GLOMERULI, INTERSTITIAL PERITUBULAR TISSUE, WALLS OF BLOOD VESSELS.
Slide 56-

56 MORPHOLOGY CONTINUED GLOMERULI - DIFFUSE OR NODULAR DEPOSITION IN THE MESANGIAL MATRIX &, BASEMENT MEMBRANE OBLITERATING THE VASCULAR LUMINA, AMORPHOUS PINK CASTS IN THE TUBULAR LUMINA.
Slide 57-

57 SPLEEN MODERATE OR MARKED ENLARGEMENT- 2 PATTERNS 1. DEPOSITS LIMITED TO SPLENIC FOLLICLES- PRODUCING TAPIOCA LIKE GRANULES ON GROSS EXAMINATION- SAGO SPLEEN.
Slide 58-

58 SPLEEN 2.DEPOSITS IN SPLENIC SINUSES,EVENTUALLY SPLENIC PULP, FORMING LARGE SHEET LIKE DEPOSITS- LARDACEOUS SPLEEN . GROSSLY DEPOSITS ARE PALE GREY WAXY
Slide 59-

59 LIVER MAY CAUSE MASSIVE ENLARGEMENT EVEN UPTO - 9000g LIVER IS PALE GREY WAXY
Slide 60-

60 LIVER MICROSCOPICALLY DEPOSIT – FIRST IN THE SPACE OF DISSE, THEN PROGRESSIVELY ENCROACH ON HEPATOCYTES & SINUSOIDS- EVENTUALLY REPLACED BY SHEETS OF AMYLOID.
Slide 61-

61 HEART SYSTEMIC OR LOCALISED. LOCALISED SENILE CARDIAC AMYLOIDOSIS DEPOSITS MAY BE MINIMAL OR MODERATE- GREY PINK DEW DROPS LIKE SUBENDOCARDIAL ELEVATIONS PERTICULARLY IN THE ATRIAL CHAMBERS
Slide 62-

62 HEART MICROSCOPICALLY – DEPOSITS ARE SEEN BETWEEN THE MYOCARDIAL FIBRES CAUSING PRESSURE ATROPHY OF MYOCARDIUM
Slide 63-

63 AMYLOIDOSIS OF KIDNEY & HEART
Slide 64-

64 OTHER ORGANS USUALLY IN SYSTEMIC DISEASES ADRENALS ,THYROID & PITUITARY ARE COMMON SITES. DEPOSITS ARE IN RELATION TO STROMA & ENDOTHELIAL CELLS CAUSING PRESSURE ATROPHY OF PARENCHYMAL CELLS WITHOUT APPARENT DISTURBANCE IN FUNCTION
Slide 65-

65 OTHER ORGANS NODULAR MASSES IN THE TONGUE MAY PRODUCE MACROGLOSSIA
Slide 66-

66 DIAGNOSIS BECAUSE OF FREQUENT INVOLVEMENT OF GIT IN SYSTEMIC CASES , GINGIVAL, INTESTINAL & RECTAL BIOPSIES ARE EMPLOYED IN THE DIAGNOSIS
Slide 67-

67 CLINICAL FEATURES 1. AMYLOIDOSIS MAY BE A AUTOPSY FINDING IN A PATIENT WHO HAD NO CLINICAL MANIFESTATION. 2. IT MAY BE RESPONSIBLE FOR SERIOUS CLINICAL DYSFUNCTION & EVEN DEATH.
Slide 68-

68 CLINICAL FEATURES WEAKNESS ,FATIGUE & WEIGHT LOSS ARE THE INITIAL SYMPTOMS
Slide 69-

69 CLINICAL FEATURES LATER AMYLOIDOSIS MANIFEST IN ONE OF SEVERAL WAYS- RENAL DISEASE , HEPATOMEGALY,SPLEENOMEGALY OR CARDIAC ABMALITIES. (RESTRICTIVE CARDIOMYOPATHY- ARRHYTHMIAS)
Slide 70-

70 DIAGNOSIS BIOPSY FOLLOWED BY CONGO RED STAIN –EXAMINATION UNDER POLARIZING MICROSCOPE 1. RECTAL ,GINGIVAL BIOPSY -75% POSITIVE.
Slide 71-

71 DIAGNOSIS 2 .ABDOMINAL FAT ASPIRATES- STAIN WITH CONGO RED IS A SIMPLE LOW RISK METHOD. IN PRIMARY AMYLOIDOSIS
Slide 72-

72 DIAGNOSIS IN PRIMARY AMYLOIDOSIS 1.URINE PROTEIN ELECTROPHORESIS & IMMUNOELECTROPHORESIS 2.BONE MARROW ASPIRATE TO SEE INCREASE IN PLASMA CELLS
Slide 73-

73 PROGNOSIS 1. GENERALISED AMYLOIDOSIS- POOR PROGNOSIS- 1- 3 YEAR SURVIVAL TIME AFTER DIAGNOSIS
Slide 74-

74 PROGNOSIS 2. IN SAA AMYLOIDOSIS PROGNOSIS DEPENDS ON THE CONTROL OF THE UNDERLYING DISEASE. 3. MYELOMA ASSOCIATED AMYLOIDOSIS- POORER PROGNOSIS